Sisebenzisa amakhukhi ukuthuthukisa ulwazi lwakho.Ngokuqhubeka nokuphequlula leli sayithi, uyavumelana nokusebenzisa kwethu amakhukhi.Ulwazi Olwengeziwe.
Abantu emhlabeni wonke basebenzisa iziphuzo zamandla ukuze bathuthukise ukugxila kwabo kanye nokukhiqiza.Enye yezindlela eziphumelela kakhulu zokuhlaziya lezi ziphuzo i-capillary electrophoresis.Lesi sihloko sihlola amandla kanye nokufaneleka uma kuqhathaniswa nezinye izindlela ezifana ne-liquid chromatography.
Iziphuzo eziningi ezinamandla zenziwe ngezinto eziqukethe i-caffeine, kuhlanganise ne-caffeine ne-glutamate.I-caffeine iyi-alkaloid evuselelayo etholakala ezinhlotsheni zezitshalo ezingaphezu kuka-63 emhlabeni jikelele.I-caffeine ehlanzekile ibabayo, ayinambitheki, iqinile emhlophe.Isisindo se-molecule ye-caffeine 194.19 g, iphoyinti elincibilikayo 2360°C.I-caffeine iyi-hydrophilic ezingeni lokushisa legumbi lokuhlushwa okuphezulu okungu-21.7 g/l ngenxa yokuphinda isebenze okumaphakathi.
Iziphuzo ezibandayo ziyizinhlelo eziyinkimbinkimbi eziqukethe izithako eziningi ezahlukene, kokubili okungaphili nemvelo.Ukuhlolwa kokuhlukaniswa kubalulekile ukuze kutholwe kahle futhi kuhlolwe ezinye izinhlobo ze-caffeine nama-benzoate.Indlela ejwayeleke kakhulu esetshenziswa ukuhlola ukuhlukaniswa okuhlangene i-liquid chromatography (LC).
I-Liquid chromatography kubikwa ukuthi isetshenziselwa ukuhlukanisa phakathi kohlu olubanzi lwama-organic molecule, kusukela kokungcolisa isisindo samangqamuzana amancane kuya kuma-peptide e-antimicrobial.Ukuxhumana okuhlukile phakathi kwezigaba ezihambayo nezimile zamamolekyuli kusampula zisekela ukuhlukaniswa kwe-chromatography ewuketshezi.Lapho isibopho siqina, i-molecule ibamba kangcono indawo yayo.
Enye indlela yezinqubo ze-HPLC ukuhlukaniswa nge-silica capillary electrophoresis encane ehlanganisiwe, esebenzisa inkambu kagesi ukuhlukanisa izinhlanganisela ezivela emaqenjini amakhemikhali ahlukene ngesampula eyodwa.I-CE ingahlukaniswa ngezindlela eziningana zokuhlukanisa kuye ngama-capillaries nama-ion asetshenzisiwe.
Indlela ye-capillary electrophoresis iwusizo kakhulu ekuhloleni ukudla nesiphuzo ngenxa yezinzuzo zayo zesampula ephansi kanye nokusetshenziswa kwe-reagent, isikhathi sokuhlaziya esifushane, izindleko eziphansi zokusebenza, ukulungiswa okuphezulu, ukusebenza kahle kokususa okuphezulu, ukulula kokuhlola kanye nokuthuthukiswa kwenqubo esheshayo.
Indlela yokuhlukanisa i-electrophoresis isekelwe ekunyakazeni okuhlukene kwama-ion amakhemikhali kuseli ye-electrolytic ngaphansi kwesenzo senkambu kagesi esetshenzisiwe.Uma kuqhathaniswa nemishini eyinkimbinkimbi ye-chromatography ye-liquid, imishini ye-capillary electrophoresis ilula ngokuyisisekelo.Ipayipi elixhumayo elinobubanzi bangaphakathi obungama-25-100 m kanye nobubanzi obungu-20-100 cm lixhuma ama-buffer cell amabili, lapho amandla afinyelela ku-voltage aphezulu (0-30 kV) anikezwa ngama-conductor kanye nesekhethi ye-electrolysis esebenzayo ilayishwa njenge- inkampani yenethiwekhi ekhokhisiwe.
Ngokuvamile, i-anode ibhekwa njenge-capillary inlet futhi i-cathode ibhekwa njenge-capillary outlet.Inani elincane lesampula lijovwa nge-hydraulically noma ngogesi ohlangothini lwe-anode lwe-capillary.Ukumnika okunenjini kwenziwa ngokufaka isitsha sebhafa esikhundleni sebhodlela lesampula kanye nokusebenzisa amandla kagesi isikhathi esithile ukuvumela izinhlayiya ukuba zingene ku-capillary.
I-Hydrostatic infusion iletha isampula ngokusekelwe ekwehleni kwengcindezi phakathi kwe-inlet ne-outlet ye-capillary, futhi inani lesampula elijovwe linqunywa ukwehla kwengcindezi nokuqina kwe-polymer matrix.Ngemva kokulayishwa kwesampula, ingxenye yesampula inqwabelana ekuvuleni kwe-capillary.
Izakhiwo zokuhlukanisa zamasu we-capillary electrophoresis zingalinganiswa ngezindlela ezimbili: ukulungiswa kokuhlukana, ama-Rs, nokusebenza kahle kokuhlukanisa.Isixazululo sabahlaziyi ababili sibonisa ukuthi bangakwazi kanjani ukuhlukanisa ngokuphumelelayo.Uma inani lama-Rs lilikhulu, kulapho inani eliphakeme livela khona.Ukulungiswa kokwehlukana kulinganisa ukusebenza kahle kokuhlukanisa futhi kuhlola ukuthi ingabe ukulungiswa endaweni yokuhlola kungaholela ekuhlukaniseni kwezingxube.
Ukusebenza kahle kokuhlukanisa i-N iyindawo ecatshangelwayo lapho izigaba ezimbili zilingana, zimelelwa inani lamaphaneli ahlukene, kuye ngekhwalithi yekholomu noketshezi.
Ucwaningo olusha olushicilelwe ku-International Conference on Agriculture and Sustainability luhlose ukuphenya ikhono le-capillary electrophoresis ukukhomba ama-nitrogenous compounds kanye ne-ascorbic acid eziphuzweni, kanye nomphumela we-electrophoresis variables on the quantitative properties of the method.
Izinzuzo ze-capillary electrophoresis ngaphezu kokusebenza okuphezulu kwe-chromatography ye-liquid zihlanganisa izindleko zocwaningo eziphansi nokuhambisana kwemvelo, kanye nokuhlolwa kwe-asymmetric organic acid noma iziqongo eziyisisekelo.I-Capillary electrophoresis inikeza ukunemba okwanele kokuhlonza amakhemikhali e-labile kumatrices ayinkimbinkimbi anemingcele ethile eyisisekelo (ukuhlakazwa kwenhlama ku-buffer ehambayo, ukuqinisekisa ukufana kokwakheka kwe-buffer, ukungaguquguquki kwezinga lokushisa kwezingqimba ezihlukanisayo).
Kafushane, nakuba i-capillary electrophoresis inezinzuzo eziningi ngaphezu kwe-chromatography yoketshezi esebenza kahle, iphinde ibe nokubi njengezikhathi zokuhlaziya ezinde.Olunye ucwaningo kufanele lwenziwe ukuthola izindlela zokuthuthukisa le ndlela.
Rashid, SA, Abdulla, SM, Najeeb, BH, Hamarashid, SH, & Abdulla, OA (2021). Rashid, SA, Abdulla, SM, Najeeb, BH, Hamarashid, SH, & Abdulla, OA (2021).U-Rashid, SA, Abdullah, SM, Najib, BH, Hamarasheed, SH, no-Abdullah, OA (2021).U-Rashid SA, u-Abdullah SM, uNajib BH, uHamarasheed SH no-Abdulla OA (2021).Ukunqunywa kwe-caffeine ne-sodium benzoate eziphuzweni zamandla ezingenisiwe nezendawo kusetshenziswa i-HPLC ne-spectrophotometer.I-IOP Conference Series: Earth and Environmental Sciences.Itholakala ku-: https://iopscience.iop.org/article/10.1088/1755-1315/910/1/012129/meta.
I-ALVES, AC, MEINHART, AD, & FILHO, JT (2019). I-ALVES, AC, MEINHART, AD, & FILHO, JT (2019).I-ALVES, AS, MEINHART, AD, ne-FILHO, JT (2019).I-ALVES, AS, MEINHART, AD ne-FILHO, JT (2019).Ukuthuthukiswa kwendlela yokuhlaziya kanyekanye kwe-caffeine ne-taurine emandleni.Isayensi Yokudla Nobuchwepheshe.Itholakala ku: https://www.scielo.br/j/cta/a/7n534rVddj3rXJ89gzJLXvh/?lang=en
Tuma, Piotr, Frantisek Opekar, and Pavel Dlouhy.(2021).I-Capillary kanye ne-microarray electrophoresis ene-non-contactivity conductivity determination for food and drink analysis.i-chemistry yokudla.131858. Itholakala ku: https://linkinghub.elsevier.com/retrieve/pii/S0308814621028648.
I-Khasanov, VV, Slizhov, YG, & Khasanov, VV (2013). I-Khasanov, VV, Slizhov, YG, & Khasanov, VV (2013).Khasanov VV, Slizhov Yu.G., Khasanov VV (2013).Khasanov VV, Slizhov Yu.G., Khasanov VV (2013).Ukuhlaziywa kweziphuzo zamandla nge-capillary electrophoresis.Ijenali ye-Analytical Chemistry.Itholakala ku: https://link.springer.com/article/10.1134/S1061934813040047.
Umlandeli, u-KK (207).Ukuhlaziywa kwe-capillary yama-preservatives eziphuzweni zamandla.I-California Polytechnic State University, ePomona.Itholakala ku: https://scholarworks.calstate.edu/concern/theses/mc87ps371.
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U-Ibtisam uphothule e-Islamabad Institute of Space Technology eneziqu ze-bachelor kwezobunjiniyela be-aerospace.Phakathi nomsebenzi wakhe wezemfundo, ubambe iqhaza ezinhlelweni eziningi zocwaningo futhi uhlele ngempumelelo imisebenzi eminingana yangaphandle kwezifundo njenge-International World Space Week kanye neNgqungquthela Yamazwe Ngamazwe Yobunjiniyela Bezindiza.U-Ibtisam uwine umncintiswano we-eseyi yolimi lwesiNgisi phakathi nezinsuku zakhe zomfundi futhi ubelokhu ekhombisa intshisekelo enkulu ocwaningweni, ekubhaleni nasekuhleleni.Ngemva nje kokuphothula iziqu, wajoyina i-AzoNetwork njengomsebenzi ozimele ukuze athuthukise amakhono akhe.U-Ibtisam uthanda ukuhamba, ikakhulukazi emaphandleni.Ubelokhu engumlandeli wezemidlalo futhi ejabulela ukubuka ithenisi, ibhola lezinyawo nekhilikithi.Uzalelwe ePakistan, u-Ibtisam unethemba lokuthi ngelinye ilanga uzohamba emhlabeni.
Abbasi, Ibtisam.(Ephreli 4, 2022).Ukuhlaziywa kweziphuzo zamandla nge-capillary electrophoresis.I-AZOM.Ibuyiselwe ngomhla ka-Okthoba 13, 2022 kusukela ku-https://www.azom.com/article.aspx?ArticleID=21527.
Abbasi, Ibtisam."Ukuhlaziywa Kweziphuzo Zamandla nge-Capillary Electrophoresis".I-AZOM.Okthoba 13, 2022.Okthoba 13, 2022.
Abbasi, Ibtisam."Ukuhlaziywa Kweziphuzo Zamandla nge-Capillary Electrophoresis".I-AZOM.https://www.azom.com/article.aspx?ArticleID=21527.(Kusuka ngo-Okthoba 13, 2022).
Abbasi, Ibtisam.2022. Ukuhlaziywa kweziphuzo zamandla nge-capillary electrophoresis.I-AZoM, ifinyelelwe ngomhla ka-13 Okthoba 2022, https://www.azom.com/article.aspx?ArticleID=21527.
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